Introducing the Patho Gene-spin™ DNA/RNA Extraction Kit, a groundbreaking product designed for the swift extraction of DNA/RNA from diverse pathogenic samples infected by viruses or bacteria, such as plasma, blood, serum, cell-free body fluids, and tissues.
Key Features:
- Uses a low concentration of chaotropic salt for enhanced lysis efficiency.
- Achieves higher lysis efficiency without additional additives.
- Ensures stable extraction of DNA/RNA from various pathogenic samples.
- Safely utilizes a non-phenol method without the need for EtOH precipitation.
- Ideal for sample extraction in forensic medicine and disease diagnosis.
The Patho Gene-spin™ Kit excels with its low concentration of chaotropic salt, offering superior lysis efficiency compared to traditional Total RNA Extraction products. It enables rapid extraction from various pathogenic samples, inducing lysis without additional additives like mercaptoethanol. Utilizing columns with silica-gel membrane technology enhances DNA/RNA extraction speed and efficiency.
The isolated DNA/RNA is suitable for clinical diagnostics, aiding in disease identification caused by viruses and bacteria in humans and animals. A versatile tool for applications like forensic medicine and disease diagnosis, the Patho Gene-spin™ Kit provides a reliable and efficient solution for researchers and clinicians.
Patho Gene-spin™ DNA/RNA Extraction Kit
Column: 50 cols
Lysis Buffer: 35ml
Binding Buffer: 35ml
Washing Buffer A: 30ml
Washing Buffer B: (Add 40ml of EtOH befure use) 10ml
Elution Buffer: 20ml
Spin Columns: (Inserted into a 2.0ml collection tubes) 50 columns
Instruction Manual: 1 sheet
Q: What is the yield of nucleic acid extraction from normal serum samples?
A: According to some literature reports, there are genome-derived substances from 500 to 1000 in serum or plasma samples derived from healthy individuals. In case of free-circulating DNA in plasma, it is known that it presents at the level of 1-100 ng/ml. This is a fairly large range, so it is practically impossible or insignificant to infer an average yield.
Q: Is it possible to extract viral nucleic acids from cell culture supernatant?
A: Yes, it can be used to extract viral nucleic acids from cell culture supernatant in case of following the protocol of this product.
Q: I want to know the yield of a small amount of DNA or RNA. Is there other way except for using A260 absorbance?
A: The Patho Gene-spin™ DNA/RNA Extraction Kit extracts whole nucleic acids present in a sample. This means that the product extracts not only nucleic acids from the pathogen in the sample but also nucleic acids from the host. Therefore, measuring the absorbance like A260 is not the preferred method. In addition, in the case of a small amount of nucleic acids less than 10 ng / μl, the range of error is large, so it is somewhat inaccurate to trust the measured value. In this case, it is preferable to perform Real-time PCR or Real-time RT-PCR or quantify fluorescent dyes binding to nucleic acids. Of course, since the detection of pathogens belongs to a qualitative study prior to quantification, it is more important to carry out fresh storage of the sample, rapid extraction and subsequent analysis, and it is not meaningful to measure the yield of extracted nucleic acids before use.
Q: Non-specific bands seem to be increased with the sensitivity of extraction. Is there a way to improve it?
A: Unlike serum and plasma samples, whole blood, buffy-coat and tissue emulsion contain large amounts of cells. The Patho Gene-spin™ DNA/RNA Extraction Kit maximizes the efficiency of nucleic acid extraction through effective dissolution of the sample. In result, overall yield is improved. In the case of samples with high cell contents, the amount of nucleic acids derived from the host is also large. Therefore, you can get clear amplification results if template usage is reduced to 1/10 level when non-specific amplification is observed during PCR analysis.